Search Results
16 results for search term 'crispr' in category Model System
Ai14 mouseModel System - [In Vivo] [Delivery Systems Initiative] [Mouse] |
Ai14 mouse (congenic)Model System - [In Vivo] [Small Animal Testing Center (SATC), Delivery Systems Initiative] [Mouse]Show Experiments (4)
Testing new LNPs (lipid nanoparticles) for delivery of Cas9 mRNA/sgRNA in adult mouse cochlea
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Ai9 mouseModel System - [In Vivo] [Small Animal Testing Center (SATC), AAV tropism, Delivery Systems Initiative] [Mouse]Show Experiments (11)
Testing gRNA sequence and gRNA scaffold modified in Ai9 mice.
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mTmG mouseModel System - [In Vivo] [Delivery Systems Initiative] [Mouse] |
mTmG mouse (congenic)Model System - [In Vivo] [Small Animal Testing Center (SATC), Delivery Systems Initiative] [Mouse]Show Experiments (7)
Delivery of unmodified, phosphorothioate (PS)-stabilized crRNA with chemically modified, PS-stabilized tracrRNA using the S10 shuttle peptide to activate the mTmG reporter in mouse brain
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HEK-293T-disrupted_GFP-mcherry-PuroModel System - [In Vitro] [Delivery Systems Initiative] [Human] |
Neuro 2AModel System - [In Vitro] [Delivery Systems Initiative] [Mouse]Show Experiments (1)
Testing newly chemically modified crRNA and tracrRNA in mouse Neuro 2A cells
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BALB/c mouseModel System - [In Vivo] [Delivery Systems Initiative] [Mouse]Show Experiments (1)
Testing Shuttle Peptides ability to deliver GFP-NLS to airway epithelia.
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HEK-293T with Ai9 transient reporter assayModel System - [In Vitro] [Delivery Systems Initiative] [Human]Show Experiments (1) |
Mouse Embryonic FibroblastsModel System - [In Vitro] [Delivery Systems Initiative] [Mouse] |
NKModel System - [In Vitro] [Delivery Systems Initiative] [Human]Show Experiments (1)
Gene editing in vitro by various peptide variants delivering Cas12a in NK cells.
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HEK-293T-disrupted_GFP with MCV-mcherry-PuroModel System - [In Vitro] [Delivery Systems Initiative] [Human] |
Hepa1-6Model System - [In Vitro] [Delivery Systems Initiative] [Mouse]Show Experiments (1)
Testing newly chemically modified crRNA and tracrRNA in mouse Hepa 1-6 cells
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TLR-MCV1 mouseModel System - [In Vivo] [Delivery Systems Initiative] [Mouse] |
HEK-293-TgEF1a-eGFP-BSDModel System - [In Vitro] [Genome Editors] [Human]Show Experiments (1)
Testing newly discovered Biggie Phage editors in human cells
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Primary Airway Epithelia (Human)Model System - [In Vitro] [Collaborative Opportunity Fund, Delivery Systems Initiative] [Human]Show Experiments (4)
Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells
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16 results for search term 'crispr' in category Model System
Type | Organism | Subtype | Name | Description | Source | View Associated.. |
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Animal | Mouse | Ai14 mouse | Ai14 mouse has a loxP-flanked STOP cassette preventing transcription of a CAG promoter-driven red fluorescent protein variant (tdTomato) - all inserted into the Gt(ROSA)26Sor locus. The att site flanked neo selection cassette has been removed in this strain. | The Jackson Laboratory | ||
Animal | Mouse | Ai14 mouse (congenic) | Ai14 mouse has a loxP-flanked STOP cassette preventing transcription of a CAG promoter-driven red fluorescent protein variant (tdTomato) - all inserted into the Gt(ROSA)26Sor locus. The att site flanked neo selection cassette has been removed in this strain. | The Jackson Laboratory |
Show Experiments (4)
Testing new LNPs (lipid nanoparticles) for delivery of Cas9 mRNA/sgRNA in adult mouse cochlea
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Animal | Mouse | Ai9 mouse | Ai9 mouse has a loxP-flanked STOP cassette preventing transcription of a CAG promoter-driven red fluorescent protein variant (tdTomato) - all inserted into the Gt(ROSA)26Sor locus. | The Jackson Laboratory |
Show Experiments (11)
Testing gRNA sequence and gRNA scaffold modified in Ai9 mice.
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Animal | Mouse | mTmG mouse | ROSAmT/mGÂ is a cell membrane-targeted, two-color fluorescent Cre-reporter allele. Prior to Cre recombination, cell membrane-localized tdTomato (mT) fluorescence expression is widespread in cells/tissues. Cre recombinase expressing cells (and future cell lineages derived from these cells) have cell membrane-localized EGFP (mG) fluorescence expression replacing the red fluorescence | The Jackson Laboratory | ||
Animal | Mouse | mTmG mouse (congenic) | mTmG is a double-fluorescent reporter transgenic mouse which expresses membrane-targeted tdTomato flanked by loxP sequences, followed by membrane-targeted GFP. After genomic cleavage by Cas9 at two sites, or Cre recombinase between loxP sites, tdTomato expression is lost and GFP is expressed. | The Jackson Laboratory |
Show Experiments (7)
Delivery of unmodified, phosphorothioate (PS)-stabilized crRNA with chemically modified, PS-stabilized tracrRNA using the S10 shuttle peptide to activate the mTmG reporter in mouse brain
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Cell | Human | Immortalized | HEK-293T-disrupted_GFP-mcherry-Puro | HEK293T cells with an integrated reporter for TLR1 reporter editing. HEK293T is an epithelial-like cell that was isolated from the kidney of a patient. | ||
Cell | Mouse | Neuroblastoma | Neuro 2A | Neuro-2a cells are mouse neuroblasts with neuronal and amoeboid stem cell morphology isolated from brain tissue. | ATCC |
Show Experiments (1)
Testing newly chemically modified crRNA and tracrRNA in mouse Neuro 2A cells
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Animal | Mouse | BALB/c mouse | BALB/cJ is a commonly used inbred. Key traits include a susceptibility to developing the demyelinating disease upon infection with Theiler's murine encephalomyelitis virus. The BALB/cJ substrain is susceptible to Listeria, all species of Leishmania, and several species of Trypanosoma, but is resistant to experimental allergic orchitis (EAO). | The Jackson Laboratory |
Show Experiments (1)
Testing Shuttle Peptides ability to deliver GFP-NLS to airway epithelia.
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Cell | Human | Immortalized | HEK-293T with Ai9 transient reporter assay | HEK-293T cells transfected with an Ai9 inducible transgene reporter plasmid used to test gene editing activity by fluorescence. HEK293T is an epithelial-like cell that was isolated from the kidney of a patient. |
Show Experiments (1) |
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Cell | Mouse | Primary cells | Mouse Embryonic Fibroblasts | Primary cell line | In-house: https://jacks-lab.mit.edu/protocols/making_mefs | |
Cell | Human | Primary cells | NK | Natural Killer cells. White blood cells; | GreenCross LabCell |
Show Experiments (1)
Gene editing in vitro by various peptide variants delivering Cas12a in NK cells.
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Cell | Human | Immortalized | HEK-293T-disrupted_GFP with MCV-mcherry-Puro | HEK293T cells with an integrated reporter for TLR-MCV1 reporter editing. HEK293T is an epithelial-like cell that was isolated from the kidney of a patient. | ||
Cell | Mouse | Hepatoma | Hepa1-6 | Stable mouse cell line of liver epithelial cells. | ATCC |
Show Experiments (1)
Testing newly chemically modified crRNA and tracrRNA in mouse Hepa 1-6 cells
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Animal | Mouse | TLR-MCV1 mouse | TLR-MCV1 transgene knocked into Rosa26 locus | |||
Cell | Human | Immortalized | HEK-293-TgEF1a-eGFP-BSD | HEK293 cells with lentiviral insertion of EF1a promoter driving expression of eGFP and SV40 promoter driving expression of BSD. HEK293 is an epithelial-like cell that was isolated from the kidney of a patient. |
Show Experiments (1)
Testing newly discovered Biggie Phage editors in human cells
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Cell | Human | Primary cells | Primary Airway Epithelia (Human) | Primary airway epithelia from non-CF donors | University of Iowa |
Show Experiments (4)
Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells
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